In vitro study of genes and molecular pathways differentially regulated by synchrotron microbeam radiotherapy

Y Yang; JC Crosbie; P Paiva; M Ibahim; A Stevenson; PAW Rogers

Journal article

In vitro study of genes and molecular pathways differentially regulated by synchrotron microbeam radiotherapy

Y Yang, JC Crosbie, P Paiva, M Ibahim, A Stevenson, PAW Rogers

Radiation Research | RADIATION RESEARCH SOC | Published : 2014

DOI: 10.1667/RR13778.1

Abstract

The aim of this study was to identify genes and molecular pathways differentially regulated by synchrotron-generated microbeam radiotherapy (MRT) versus conventional broadbeam radiotherapy (CRT) in vitro using cultured EMT6.5 cells. We hypothesized (based on previous findings) that gene expression and molecular pathway changes after MRT are different from those seen after CRT. We found that at 24 h postirradiation, MRT exerts a broader regulatory effect on multiple pathways than CRT. MRT regulated those pathways involved in gene transcription, translation initiation, macromolecule metabolism, oxidoreductase activity and signaling transduction in a different manner compared to CRT. We also fo..

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University of Melbourne Researchers

Yuqing Yang Author

Peter Rogers Author

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OPTIMISING SYNCHROTRON MICROBEAM RADIATION THERAPY FOR CANCER TREATMENT

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Grants

Awarded by Australian National Health and Medical Research Council

Funding Acknowledgements

Research was supported by the Australian National Health and Medical Research Council grant 606614. We are grateful to Associate Professor Robin Anderson for providing EMT6.5 cell line. We thank Drs. Chris Hall, Trevor Ackerly and Frank Gagliardi for assistance with operating the IMBL.